Magnaporthe oryzae Methods and Protocols

This volume highlights molecular methods to study the phytopathogenic rice blast fungus Magnaporthe oryzae. Chapters in this book cover the history, development, and evolution of the pathogen; molecular methods to increase the knowledge of the biology, genetic, and metabolic diversity of the pathogen; and the pathogen’s adaptability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Magnaporthe oryzae: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this developing field. 

The Impact of Blast Disease: Past, Present, and Future.- The Biology of Invasive Growth by the Rice Blast Fungus Magnaporthe oryzae.- Analysis of the Secondary Metabolism in Magnaporthe oryzae.- The Determination of Carbohydrates by High-Performance Anion-Exchange Chromatography Coupled with Pulsed Amperometric Detection (HPAEC-PAD).- Characterizing the Secretion Systems of Magnaporthe oryzae.- Specimen Preparation and Observations of Magnaporthe oryzae Appressorial Cells Under Electron Microscopy.- 4D Widefield Fluorescence Imaging of Appressorium Morphogenesis by Magnaporthe oryzae.- Tandem Affinity Purification (TAP) of Low Abundance Protein Complexes in Filamentous Fungi Demonstrated using Magnaporthe oryzae.- Quantitative Proteome and Phosphoproteome Profiling in Magnaporthe oryzae.- A Target-Based In Vivo Test System to Identify Novel Fungicides with Mode of Action in the HOG Pathway.- Making Visualization Work for You: Deriving Valuable Insights from Omics Data.- Genome Editing using CRISPR/Cas9 System in the Rice Blast Fungus.- Diurnal, Circadian, and Photomorphogenic Analyses in Magnaporthe oryzae.- Methods to Study Autophagocytosis in Magnaporthe oryzae.- Molecular Detection of Magnaporthe oryzae from Rice Seeds.- Isolation and Functional Analysis of Effector-Proteins of Magnaporthe oryzae.- Analysis of Genetic Variations and Genomic Instabilities in Magnaporthe oryzae.- Controllable Bypass Suppression in Magnaporthe oryzae.