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Lung Cancer Methods and Protocols




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Dettagli

Genere:Libro
Lingua: Inglese
Editore:

Humana

Pubblicazione: 03/2021
Edizione: 1st ed. 2021





Trama

This detailed book serves as a laboratory manual containing vital protocols and in-depth discussion involving commonly used experimental approaches for the characterization of several aspects of lung tumor biology. Beginning with an extensive section on biomarker detection, the volume continues with chapters on the genetic and molecular characterization of lung cancer biological samples as well as protocols for the generation of research tools and pre-clinical lung cancer models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Lung Cancer: Methods and Protocols provides a global perspective of research efforts related to lung cancer, while allowing researchers to experimentally probe the different aspects of lung cancer research, including the experimentally relevant tests used in the establishment of lung cancer diagnosis and prognosis, in their laboratories.




Sommario

1. Automated TTF-1 Immunohistochemistry Assay for the Differentiation of Lung Adenocarcinoma Versus Lung Squamous Cell Carcinoma

            Rosa Vélez Cintrón, Andrés J. Martínez, JoAnn Jusino, María Conte-Miller, and Adalberto Mendoza

 

2. Immunohistochemical Detection of p40 Expression in Lung Cancer Clinical Samples

            Aruna Nambirajan and Deepali Jain

 

3. Automated Immunohistochemistry Assay for the Detection of Napsin-A in Formalin-Fixed Paraffin-Embedded Tissues from Lung Tumors

            Rosa Velez Cintrón, JoAnn Jusino, María Conte Miller, Andrés J. Martínez, and Adalberto Mendoza

 

4. Detection of Programmed Cell Death Ligand 1 Expression in Lung Cancer Clinical Samples by an Automated Immunohistochemistry System

            Edwin Roger Parra and Sharia Hernández Ruiz

 

5. Western Blot as a Support Technique for Immunohistochemistry to Detect Programmed Cell Death Ligand 1 Expression

            Edwin Roger Parra and Sharia Hernández

 

6. Creation of Formalin-Fixed, Paraffin-Embedded 3D Lung Cancer Cellular Spheroids for the Optimization of Immunohistochemistry Staining Procedures

            Jennifer Cabán-Rivera, Camille Chardón-Colón, Alberto Pedraza-Torres, Yoan E. Rodríguez, Raymond Quiñones-Alvarado, and Pedro G. Santiago-Cardona

 

7. Immunoblot Validation of Phospho-Specific Antibodies Using Lung Cancer Cell Lines

            Wilfredo M. Pedreira-García, Jaileene Pérez-Morales, Camille Chardón-Colón, Jennifer Cabán-Rivera, and Pedro G. Santiago-Cardona

 

8. Detection of Non-Small Lung Cell Carcinoma-Associated Genetic Alterations Using a NanoString Gene Expression Platform Approach

            Johan Staaf, Mats Jönsson, and Anna F. Karlsson

 

9. A PCR-Based Approach for Driver Mutation Analysis of EGFR, KRAS, and BRAF Genes in Lung Cancer Tissue Sections

            Rodrigo de Oliveira Cavagna, Leticia Ferro Leal, Flávia Escremim de Paula, Gustavo Noriz Bernardinelli, and Rui Manuel Reis

 

10. Six-Color CrystalTM Digital PCR for the High-Plex Detection of EGFR Mutations in Non-Small Cell Lung Cancer

            Jordan Madic, Cécile Jovelet, Imane Dehri, and Allison C. Mallory

 

11. Detection of MET Exon 14 Skipping Alterations in Lung Cancer Clinical Samples Using a PCR-Based Approach

            Jane S.Y. Sui, Stephen P. Finn, and Steven G. Gray

 

12. Immunocytochemical Detection of ALK and ROS1 Rearrangements in Lung Cancer Cytological Samples

            Diane Frankel, Elise Kaspi, and Patrice Roll

 

13. A Method for the Establishment of Human Lung Adenocarcinoma Patient-Derived Xenografts in Mice

            Joanne Lundy, Brendan J. Jenkins, and Mohamed I. Saad

 

14. A Method for the Establishment and Characterization of Mouse Lung Adenocarcinoma Cell Lines that Mimic Traits of Human Adenocarcinomas

            Magda Spella, Ioannis Lilis, and Georgios T. Stathopoulos

 

15. Whole Transcriptome Sequencing Analysis of Cancer Stem/Progenitor Cells Obtained from Mouse Lung Adenocarcinomas

            Ansam Sinjab, Reem Daouk, Wassim Abou-Kheir, and Humam Kadara

 

16. In Vivo Imaging of Orthotopic Lung Cancer Models in Mice

            Peng Liu, Liwei Zhao, Laura Senovilla, Oliver Kepp, and Guido Kroemer

 

17. An Annexin V- FITC - Propidium Iodide-Based Method for Detecting Apoptosis in a Non-Small Cell Lung Cancer Cell Line

            Robin Kumar, Ankit Saneja, and Amulya K. Panda

 

18. Detection of DNA Adduct Formation in Rat Lungs by a Micro-HPLC/MS/MS Approach

            Angélica B. Sanchez, Camila C.M. Garcia, Paolo Di Mascio, and Marisa H.G. Medeiros

 

19. A Method for Liposome Co-Encapsulation of Phenethyl Isothiocyanate and Cisplatin and Determining its Toxicity against Lung and Lung Cancer Cells

            Mengwei Sun and Anthony J. Di Pasqua











Altre Informazioni

ISBN:

9781071612774

Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 254 x 178 mm Ø 714 gr
Formato: Copertina rigida
Illustration Notes:XIV, 258 p. 45 illus., 43 illus. in color.
Pagine Arabe: 258
Pagine Romane: xiv


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