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Integrated Virus Detection




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Dettagli

Genere:Libro
Lingua: Inglese
Editore:

CRC Press

Pubblicazione: 11/2014
Edizione: 1° edizione





Note Editore

Multiple viruses can be detected concurrently using the Integrated Virus Detection System (IVDS). Integrated Virus Detection describes this technology and provides many examples of applications including a chapter on viruses found in honeybees with descriptions of seasonal and yearly variation. This straightforward technology can be used to detect known, unknown, and unsequenced viruses collected from environmental and other complex biological sources. This book summarizes more than ten US patents issued for the invention of the IVDS, which is the common name of the electrospray–differential mobility analyzer method. The IVDS is powering mankind’s ability to rapidly detect, measure, and monitor viruses as well as virus-like particles. Three facts make rapid detection possible: virus size, which ranges from 20 to 800 nm.; disparity in each virus species’ particle size thus allowing size data to be used for detection and preliminary identification; and the fact that virus particle density is distinct from other nanoparticles. The IVDS can ascertain the absence of virion particles, thus presenting compelling evidence of a true negative, which is useful in verifying decontamination and other processes. In addition, large numbers of samples may be processed in an automated fashion, providing an excellent means to prescreen them for judicious targeting of subsequent tests such as PCR or the discriminating method for identifying microbes, which is mass spectrometry proteomics.* The book is helpful to anyone interested in virus detection, especially in situations where many viral types may coexist. *Identifying Microbes by Mass Spectrometry Proteomics (CRC Press 2013)




Sommario

Detection of Whole VirusesIntroductionHistorical BackgroundIt Works! The Eureka MomentFlowchart for Virus DetectionConcept of IVDS OperationIVDS Systems and OperationsOrganization of BookInstrumentationIntroductionDescriptions of IVDS ComponentsIVDS-BetaCommercial IVDSRapidDx-IVDSCapillary Diameter Effect and IVDS StabilityElectrospray StabilityProof Concept Using MS2IntroductionPhysical Basis of InventionMS2 as Test VirusCharacterization of MS2 Using IVDSRemoving Complex Growth MediaFiltration Characteristics of MS2 BacteriophageSummaryConcentration of VirusesIntroductionConcentrator DeviceCause and Effect CurvesIntroductionEffects of Temperature on MS2 SurvivalDose Response Curves for MS2 (High pH) Dose Response Curves for MS2 (Low pH)Isolating Single Viruses from Multivirus MixturesIntroductionProcedureDifferential Mobility SeparationConclusionsSummaryAnalyzing Various VirusesIntroductionIVDSSample Analysis ProceduresAnalyses of and Results for Specific VirusesAnalysis of Complex MediaWater Sample AnalysisRecovery of Viruses from SurfacesDetection of Viruses in Bark BeetlesDetection of Other NanoparticlesIntroductionParticle Size Results: Comparison of Malvern Nano ZSDirect Counting of PiliIntroductionDetecting PiliTesting ProceduresConclusionsDetecting Viruses in HoneybeesIntroduction and Formation of BVS, Inc.Detection of Viruses from Single HoneybeeRosetta Stone ProjectHoneybee Nutrition StudyEssential Oils StudySnapshots of Viral Loads in HoneybeesConclusionsPulsed Lamp DecontaminationIntroductionMethodsResultsDiscussion and ConclusionsCurrent and Future TrendsIntroductionPotential Applications of IVDSNew RapidDx InstrumentThe Future




Autore

Charles H. Wick is a retired lieutenant colonel and senior scientist from the U.S. Army Edgewood Chemical Biological Center. He earned his PhD from the University of Washington. Dr. Wick is best known for work in forensic science done in concert with the Department of Defense. Dr. Wick’s research work has resulted in international recognition as an authority on individual performance for operations conducted on a nuclear, biological, and chemical battlefield. He has made lasting and important contributions to forensic science and antiterrorism and holds several U.S. patents in the area of microbe detection and classification. He has written more than 45 civilian and military publications and has received various awards and citations.










Altre Informazioni

ISBN:

9781482230055

Condizione: Nuovo
Dimensioni: 9.2 x 6.1428571 in Ø 1.10 lb
Formato: Copertina rigida
Illustration Notes:184 b/w images and 32 tables
Pagine Arabe: 255


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