Sommario
Part I: Overview of Immunity
1 Immunity
2 Components of immunity
3 Hematopoiesis
4 The Immune system: pathogen sensing and detection
5 Lymphoid organs
6 Innate and Adaptive immunity
7 Leukocyte homing, migration and recirculation
8 Apoptosis
9 Antigens
10 B cell development, activation and immunoglobulin structure
11 Immunoglobulin and T cell receptor gene rearrangements
12 Major histocompatibility complex proteins
13 T cell development and T cell receptor structure
14 Cytokines
15 Lymphocyte signals
16 Complement fixation
17 Cell mediated immunity
18 Hypersensitivities
19 Parasite Immunity
20 Vaccines
21 Transplantation
22 Immune regulation and autoimmunity
23 Immunodeficiencies
24 Cancer
25 Practice questions
26 Study guide for exam 1
Part II: Laboratory Manual
27. Immunology laboratory policies
28. Instructions for notebook entries
29. Instructions for writing lab reports
30. Lab report topics
EXERCISE 1: Using a compound microscope
EXERCISE 2: Pipetting and dilution technique, identification of mouse lymphoid organs
EXERCISE 3: Blood Cell Preparation, Leukocyte differentiation and ABO Blood Typing
EXERCISE 4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and Identification of specific cells using surface antigens
EXERCISE 5: Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial cells
EXERCISE 6: Clearance of bacteria from mouse blood by the RES
EXERCISE 7: Hemolytic Plaque assay (The Jeme Plaque Assay)
EXERCISE 8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and screening assays
EXERCISE 9: Determination of Antibody Titer: Screening Techniques
EXERCISE 10: Immunodiffusion and Immunoelectrophoresis
EXERCISE 11: Protein Affinity Purification of Rabbit IgG
EXERCISE 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
EXERCISE 13: Immunoblotting (Western Blotting)
EXERCISE 14: Protein Assay
EXERCISE 15: Enzyme-linked Immunosorbent Assay (ELISA)
EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT)
EXERCISE 17: Immunofluorescence assay (IFA)
EXERCISE 18: Flow Cytometry
EXERCISE 19: Complement Fixation Assays
Tissue Cell Culture Techniques
EXERCISE 20: Mitogen Induced Response of Lymphocytes:
Detection of Band T cell Lymphocytes Using Immunofluorescence
EXERCISE 21: Induction of IL-2 Secretion from Mouse Lymphoma Cells
EXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation for Antibody Production
EXERCISE 23: Macrophage Migration Inhibition
EXERCISE 24: Monoclonal Antibody Preparation
EXERCISE 25: Immunization of mice with a recombinant protein
EXERCISE 26: Polarization of T helper cells into TH1 subset
EXERCISE 27: Immunoprecipitation
EXERCISE 28: Induction of apoptosis in T lymphocytes and detection of apoptotic markers
EXERCISE 29: Cloning and sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal antibodies
Appendix
Sample Practice Questions
