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Immunochemical Protocols




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Dettagli

Genere:Libro
Lingua: Inglese
Editore:

Humana Press

Pubblicazione: 11/2010
Edizione: Softcover reprint of hardcover 3rd ed. 2005





Trama

Immunochemical techniques have been in use for many years with early examples of bacterial strain typing dating back to the 1940s. The basis for the science is the exquisite elegance of the mammalian immune system with its ability to recognize foreign proteins and to manufacture antibody m- ecules that strongly bind to the substances that elicited them. Not only are potentially harmful pathogens and toxins recognized by the immune system, but the system can be persuaded to manufacture antibodies to an astonishing array of substances. In the early days of this science, all antibodies for investigative work were produced by immunizing mammals with the substance of interest, followed by regular donor bleeds that yielded antisera. Serum produced in this way yields heterogenic populations of antibody molecules recognizing different epitopes on the target protein, which may be adequate for its intended p- poses, but can also cause problems of crossreactivity. In 1975, Kohler and Milstein reported that spleen cells from immune donor animals could be immortalized, cloned from single cells, and grown in continuous culture. This original work described the method for the production of monoclonal antibodies.




Sommario

Immunization Strategies for Antibody Production Robert Burns Synthetic Peptides as Antigens for Antibody Production David C. Hancock and Nicola J. O'Reilly Production of Polyclonal Antibodies in Rabbits David C. Hancock and Nicola J. O'Reilly Making Hybridomas Robert Burns Growing Hybridomas Gary Entrican and Gareth Young Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments Michael Hust and Stefan Dübel Purification of Antibodies and Preparation of Antibody Fragments Graham D. Kemp Conjugation of Antibodies to Alkaline Phosphatase G. Brian Wisdom Conjugation of Antibodies to Horseradish Peroxidase G. Brian Wisdom Conjugation of Antibodies to Fluorescein or Rhodamine G. Brian Wisdom Conjugation of Antibodies to Biotin James D. Hirsch and Rosaria P. Haugland Preparation of Colloidal Gold Probes David Hughes Immunogold Probes in Light Microscopy David Hughes Immunoelectron Microscope Techniques in Plant Virus Diagnosis Daphne M. Wright Dual-Antibody Sandwich Enzyme-Linked Immunosorbent Assay William Jordan Competitive Enzyme-Linked Immunosorbent Assay William Jordan Immunoblotting Techniques Barbara Magi and Sabrina Liberatori Epitope Mapping Glenn E. Morris Generation and Testing of Immunoaffinity Columns Weilin L. Shelver Immunocapture-Polymerase Chain Reaction Vincent Mulholland Magnetic Cell Sorting Maciej Zborowski and Jeffrey J. Chalmers Cell Enrichment and Immunochemical Staining Oscar R. Lara and Jeffrey J. Chalmers Index










Altre Informazioni

ISBN:

9781617374661

Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 229 x 152 mm Ø 484 gr
Formato: Brossura
Illustration Notes:22 Illustrations, black and white
Pagine Arabe: 318
Pagine Romane: x


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