HPLC in Nucleic Acid Research Methods and Applications

This book gives the pertinent information on the high pressure liquid chromatography (HPLC) analyses of all the compounds of interest in nucleic acid metabolism. It aids chromatographers, biochemists, biomedical researchers, and chemists by giving information on applications of HPLC technique.

Part I: Overview 1. Nucleic Acid Research 2. Structures, Properties, and Chromatographic Behavior of Nucleic Acid Constituents Part II: Methodology 3. Sample Preparation 4. Chromatography 5. Peak Identification 6. Quantitative Analysis in Liquid Chromatography 7. Microbore Columns 8. Detection Systems 9. Mobile Phase Control Part III: Applications 10. Nucleic Acids 11. Oligonucleotides 12. Free Nucleotides in Biological Samples 13. Free Nucleosides and Their Bases in Physiological Fluids 14. Cyclic Nucleotides 15. Enzyme Assays by HPLC 16. Nucleotide Coenzymes 17. HPLC Analysis of Pyrimidine and Purine Anti-metabolite Drugs 18. Central Nervous System Drugs (Methylxanthine Drugs) 19. Nucleic Acid Constituents in Disease Processes