Histochemistry Of Single Molecules - Pellicciari Carlo (Curatore); Biggiogera Marco (Curatore) | Libro Humana 02/2017 - HOEPLI.it

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pellicciari carlo (curatore); biggiogera marco (curatore) - histochemistry of single molecules

Histochemistry of Single Molecules Methods and Protocols


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Lingua: Inglese


Pubblicazione: 02/2017
Edizione: 1st ed. 2017


This detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy. More in detail, the book is divided into six sections covering a variety of chemical targets.  It begins with a section on vital histochemistry and continues with chapters on histochemistry as it relates to lectins, proteins, lipids, DNA and RNA, as well as plants. The volume also contains four overview chapters on vital histochemistry, lectin histochemistry, and DNA  fluorochromes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Histochemistry of Single Molecules: Methods and Protocols aims to effectively help scientists in very different research fields to elucidate biological issues though a unique approach to molecular biology in situ.


Part I: Vital Histochemistry


1. Single Cell Cytochemistry Illustrated by the Demonstration of Glucose-6-Phosphate Dehydrogenase Deficiency in Erythrocytes

            Anna L. Peters and Cornelis J.F. van Noorden


2. Autofluorescence Spectroscopy for Monitoring Metabolism in Animal Cells and Tissues

            Anna C. Croce and Giovanni Bottiroli


3. Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree

            Vittorio Bertone, Eleonora Tarantola, and Isabel Freitas


4. Histochemical Demonstration of Tripeptidyl Aminopeptidase I

            Mashenka B. Dimitrova, Dimitrinka Y. Atanasova, and Nikolai E. Lazarov


5. Enzyme Histochemistry for Functional Histology in Invertebrates

            Francesca Cima


Part II: Lectin Histochemistry


6. Lectin Histochemistry: Historical Perspectives, State of the Art, and the Future

            Susan A. Brooks


7. Isolation of Viable Glycosylation-Specific Cell Populations for Further In Vitro or In Vivo Analysis Using Lectin-Coated Magnetic Beads

            Ellie-May Beaman, David R.F. Carter, and Susan A. Brooks


8. Lectin Histochemistry for Metastasizing and Non-Metastasizing Cancer Cells

            Gerrit Wolters-Eisfeld and Udo Schumacher


9. The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium

            Vicente Seco-Rovira, Ester Beltrán-Frutos, Jesús Hernández-Martínez, Concepción Ferrer, and Luis Miguel Pastor


Part III: Protein Histochemistry


10. Heat-Induced Antigen Retrieval in Immunohistochemistry: Mechanisms and Applications

            Shuji Yamashita and Osamu Katsumata


11. Detecting Neuronal Differentiation Markers in Newborn Cells of the Adult Brain

            Sara Bonzano and Silvia De Marchis


12. Characterizing Satellite Cells and Myogenic Progenitors During Skeletal Muscle Regeneration

            Nicolas A. Dumont and Michael A. Rudnicki


13. Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin Fixed and Paraffin Embedded Tissue

            Sabina Berezowska and José A. Galván


Part IV: Lipid Histochemistry


14. Tissue Fixation and Processing for the Histological Identification of Lipids

            Víctor Carriel, Fernando Campos, José Aneiros-Fernández, and John A. Kiernan


15. Staining Methods for Normal and Regenerative Myelin in the Nervous System

            Víctor Carriel, Antonio Campos, Miguel Alaminos, Stefania Raimondo, and Stefano Geuna


16. Nile Red Staining of Neutral Lipids in Yeast

            Kerry Ann Rostron and Clare Louise Lawrence


17. Staining of Lipid Droplets with Monodansylpentane

            Bo-Hua Chen, Huei-Jiun Yang, He-Yen Chou, Guang-Chao Chen, and Wei Yuan Yang


Part V: Nuclear Histochemistry


18. Fluorochromes for DNA Staining and Quantitation

            Giuliano Mazzini and Marco Danova


19. Osmium Ammine for Staining DNA at Electron Microscopy

            Irene Masiello and Marco Biggiogera


20. DNA Labeling at Electron Microscopy

            Nicolas Thelen and Marc Thiry


21. Visualizing RNA at Electron Microscopy by Terbium Citrate

            Marco Biggiogera and Irene Masiello


22. Two-Tailed Comet Assay (2T-Comet): Simultaneous Detection of DNA Single and Double Strand Breaks

            Elva I. Cortés-Gutiérrez, José Luis Fernández, Martha I. Dávila-Rodríguez, Carmen López-Fernández, and Jaime Gosálvez


Part VI: Plant Histochemistry


23. Detection of Endogenous Nuclear Proteins in Plant Cells: Localizing Nuclear Matrix Constituent Proteins (NMCPs), the Plant Analogues of Lamins

            Malgorzata Ciska and Susana Moreno Díaz de la Espina


24. Histochemical Analysis of Plant Secretory Structures

            Diego Demarco


25. A Histochemical Technique for the Detection of Annonaceous Acetogenins

            Guillermo Laguna-Hernández, Alicia Enriqueta Brechú-Franco, Iván De la Cruz-Chacón, and Alma Rosa González- Esquinca

Altre Informazioni



Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 254 x 178 mm Ø 8196 gr
Formato: Copertina rigida
Illustration Notes:11 Illustrations, black and white
Pagine Arabe: 341
Pagine Romane: xiv

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