Helicobacter Pylori

This book brings together a collection of methods for the successful detection, culture and investigation of H. pylori, and its associated diseases.  Chapters guide the reader through overviews on H. pylori infection, protocols for analyzing human clinical samples, and protocols for characterizing H. pylori, its functional components and its interactions with host cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Helicobacter pylori: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.

1. An Overview of Helicobacter pylori Infection

Rebecca FitzGerald and Sinéad Smith

2. The Urea Breath Test for the Non-Invasive Detection of Helicobacter pylori

Anthony O’Connor

3. Real-time Diagnosis of Helicobacter pylori during Endoscopy by Gastric Juice Analysis

Cristiano Spada and Stefania Piccirelli

4. Molecular Detection of Antibiotic-resistant Helicobacter pylori

Denise Brennan, Colm O’Morain, Deirdre McNamara, and Sinéad Smith

5. Endoscopic Detection of Helicobacter pylori by the Rapid Urease Test

6. Culture-based Antimicrobial Susceptibility Testing for Helicobacter pylori

Philippe Lehours and Francis Mégraud

7. Purification of Total RNA from Stomach Tissue Biopsies

Rebecca FitzGerald, Malini Devapal, John J. Hickey, Dermot Kelleher, and Sinéad Smith

8. Gene Expression Analysis by Reverse Transcription Quantitative PCR

Eva M. Campion and Sinéad T. Loughran

9. A Method to Investigate the Helicobacter pylori-associated DNA Methylome

Hae Dong Woo and Zdenko Herceg

10. Profiling the Gastrointestinal Microbiota

11. Helicobacter pylori Virulence Factor Genotyping

Rebecca FitzGerald, Chavi Sinha, Abbas Yadegar, and Sinéad Smith

12. Generation of Isogenic Mutant Strains of Helicobacter pylori

Stephanie L. Servetas, Jeannette M. Whitmire, and D. Scott Merrell

13. Isolation of Outer Membrane Vesicles from Helicobacter pylori

 Henry J. Windle

14. A Mouse Model of Helicobacter pylori Infection

Tanmoy Kumar Dey, Bipul Chandra Karmakar, Avijit Sarkar, Sangita Paul, and Asish Kumar Mukhopadhyay

15. Investigating the Interaction of Helicobacter pylori with the Gastric Mucosa

Marguerite Clyne, Ciara Dunne, and Brendan Dolan

16. Evaluating Host Responses to Helicobacter pylori using ELISA and Western Blot

Ciara Nulty, Gillian Barber, Ewelina Flis, and Emma M. Creagh

17. Analysis of Innate Immune Responses to Helicobacter pylori

Michelle Chonwerawong and Richard L. Ferrero

18. Analysis of T Cell Responses to Helicobacter pylori Infection

Kazuyo Kaneko and Karen Robinson