Hairy Root Cultures Based Applications Methods and Protocols

The book discusses the various methods and protocols available in hairy root culture-based research. The utilization of Agrobacterium mediated genetic transformation and establishment of hairy root cultures has paved the way for large-scale secondary metabolite production in medicinal plants. Presenting recent research and offering insights from eminent research groups, the book covers a range of topics related to hairy root-based applications, including (i) establishment of hairy roots and native production of SM (ii) yield enhancement strategies for increased SM production, like elicitation (iii) hairy roots as a tool for value-added applications such as plant-microbe interaction, characterization of plant genes and root biology studies. As such it is an informative guide and experimental manual for researchers in diverse fields of plant biology.

Chapter 1. Hairy Roots Biotechnology Unzipped: A Journey of Reality and Promises.- Chapter 2. Hairy Roots as a Source of Tropane Alkaloids.- Chapter 3. Induction, Metabolite Analysis and Transgenesis of Hairy Roots From Coleus Forskohlii.- Chapter 4. Establishment of Hairy Roots of Endangered Himalayan Plantswertiachirata: A Sustainable Alternative to Extraction from Nature.- Chapter 5. A Protocol for the Selection of Spontaneous Variants From Established Catharanthusroseus Hairy Root Cultures.- Chapter 6. Strategies for the Optimization of Culture Conditions for Increasing Metabolite Production Through Hairy Root Cultures. Monoterpenoid Indole Alkaloid Production in Catharanthus  Roseus Hairy Roots.- Chapter 7. Protocol for Enhanced Withaferin-a Production in Elicited Withania Somnifera (l.)Dunal Hairy Root Cultures.- Chapter 8. Establishment of Hairy Root Cultures of Pentalinonandrieuxii for the Production of Betulinic Acid.- Chapter  9. Tropane Alkaloid Production by the Establishment Of Hairy Root Cultures of Brugmansia Candida and Elicitation.- Chapter 10. Elicitation as an Essential Strategy For Enhancing Anthraquinone Accumulation In Hairy Root Cultures of Rubia Tinctorum.- Chapter 11. Establishment of Hairy Root Cultures for the Production of Biopharmaceuticals and Optimization of Methods for Recombinant Protein Secretion In The Culture Medium.- Chapter 12. An Improved Protocol for Agrobacterium Rhizogenes-Mediated Transformation of Recalcitrant Plants for Root Biology Studies: A Case Study of Tea Plants (Camellia Sinensis Var. Sinensis).- Chapter 13 Functional Analysis of Plant Genes Related to Arbuscular Mycorrhiza Symbiosis Using Agrobacterium Rhizogenes-Mediated Root Transformation and Hairy Root Production.- Chapter 14.Functional Characterization of Genes Involved in Legume Nodulation Using Hairy Root Cultures.- Chapter  15.  Fluidigm Biomark Diagnostic Panel for Analysis of the Expression of Nicotiana Tabacum Genes, Associated with Alkaloid Synthesis. 

Dr. Vikas Srivastava is an Assistant Professor at the Department of Botany, Central University of Jammu, India. He completed his Ph.D. at Lucknow University, India, and his post-doc at the National Institute of Plant Genome Research (NIPGR), New Delhi, India. Specializing in various areas of biotechnology, he has training and experience in diverse fields of plant biology. He has published numerous articles in respected international journals/books of repute, and has been working as Principal Investigator for a major project sponsored by the University Grant Commission (UGC), New Delhi. He is a recipient of various awards and prestigious fellowships, and has delivered several invited lectures at leading national institutes.

Dr. Shakti Mehrotra is a consulting scientist at the Department of Biotechnology, Institute of Engineering and Technology, Dr. Abdul Kalam Technical University, Lucknow, India. She completed her Ph.D. at Lucknow University, India, and post-doc at the Institute of Engineering and Technology, Lucknow (DBT-PDF) and CSIR-CIMAP (DSTYoung Scientist). Specializing in various areas of biotechnology, she has training and experience in diverse fields of plant biotechnology. She has published numerous articles in respected national and international journals/books and worked as Principal Investigator for major projects sponsored by the Department of Science and Technology (DST) and Department of Biotechnology (DBT), Government of India. She is a recipient of various awards and prestigious fellowships. She has delivered several presentations at conferences and workshops.

Dr. Sonal Mishra is currently working as a Dr. D. S. Kothari-Post Doctoral Fellow (UGC-DSKPDF) at the School of Biotechnology, University of Jammu, India. She has recently completed a SERB-National Post Doc Fellowship (SERB-NPDF) as Principal Investigator at the School of Biotechnology, University of Jammu, India. Further, she completed a post-doc at Jawaharlal Nehru University and National Institute of Plant Genome Research (NIPGR), India. She pursued her Ph.D. jointly from the Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP, Lucknow) and Jawaharlal Nehru University, New Delhi. Specializing in various areas of biotechnology, she has training and experience in diverse fields of plant biotechnology and molecular biology. She has published numerous articles in respected international journals/books. She is a recipient of various awards and prestigious fellowships, and has presented her work in at seminars and conferences.