Glycovirology Methods and Protocols

This volume details applications in molecular biological techniques and focuses on applications to determine the involvement of glycans in virus interactions. Chapters guide readers through glycan analysis, glycan distribution analysis, glycan and lectin microarray, preparation of recombinant viral domain protein, reverse genetics and receptor binding, virus-host interactions and receptor binding, and sialidase: assays and inhibitors. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Glycovirology: Methods and Protocols aims to be an essential resource for researchers who wish to learn more about glycovirology.

 

1. Comprehensive cellular glycan profiling of glycoproteins and glycosphingolipids by glycoblotting and BEP methods

Hisatoshi Hanamatsu and Jun-ichi Furukawa

 

2. High-performance liquid chromatography mapping method for the structural analysis of N-glycans at the molecular, cellular, and tissue levels

Taiki Saito and Hirokazu Yagi

 

3. Detection of sialic acids on the cell surface using flow cytometry

Hirohito Ishigaki and Yasushi Itoh

 

4. Lectin Staining to Detect Human and Avian influenza Virus Receptors in the Airway of Nonhuman Primates

Misako Nakayama and Yasushi Itoh

 

5. Preparation of Glycan Arrays Using Glycopeptides Derived from Biomaterials

Shin-ichi Nakakita, Yukari Nakakita, Ryohsuke Kurihara, and Jun Hirabayashi

 

6. Glycan profiling of viral glycoproteins with the lectin microarray

Takahiro Hiono and Atsushi Kuno

 

7. Preparation of recombinant PA endonuclease domain protein of influenza A virus and its application for glycobiology research

Takashi Kuzuhara

 

8. Receptor-binding specificity of influenza viruses

Masaki Imai, Kosuke Takada, and Yoshihiro Kawaoka

 

9. Functional analysis of sulphatide in influenza A virus infection and replication

Tadanobu Takahashi, Yuuki Kurebayashi, and Takashi Suzuki

 

10. Quantification of receptor-association, -dissociation and NA-dependent motility of influenza A particles by biolayer interferometry

Erik de Vries, Hongbo Guo, Wenjuan Du, Mengying Liu, Frank J. M. van Kuppeveld and Cornelis A.M. de Haan

 

11. Receptor-binding assay for avian influenza viruses

Takahiro Hiono and Daiki Kobayashi

 

12. Characterization of influenza virus binding to receptors on isolated cell membranes

Mikhail N. Matrosovich and Alexandra S. Gambaryan

 

13. Characterization of human parainfluenza virus receptor using terminal sialic acid linkage-modified cells

Keijo Fukushima, Tadanobu Takahashi, and Takashi Suzuki

 

14. Solid-phase binding assay for ganglioside-binding of human respiroviruses

Takashi Suzuki

 

15. Evaluation of the glycan-binding and esterase activities of hemagglutinin-esterase-fusion glycoprotein from influenza D virus

Hao Song and George F. Gao

 

16. Roles of glycans and non-glycans on the epithelium and in the immune system in H1-H18 influenza A virus infections

Nongluk Sriwilaijaroen and Yasuo Suzuki

 

17. Roles of sialyl glycans in HCoV-OC43, HCoV-HKU1, MERS-CoV and SARS-CoV-2 infections

Nongluk Sriwilaijaroen and Yasuo Suzuki

 

18. Enzymatic substrates and fluorescence imaging of influenza virus sialidase

Yuuki Kurebayashi, Tadanobu Takahashi, and Takashi Suzuki

 

19. Assays for determining the sialidase activity of influenza viruses and monitoring influenza virus susceptibility to neuraminidase inhibitors

Emi Takashita

 

20. Synthesis and Neuraminidase Inhibitory Activity of Sialic Acid Analogues with Fluoro, Phosphono and Sulfo Functionalities

Christopher J. Vavricka, Nongluk Sriwilaijaroen, Yasuo Suzuki and Hiromasa Kiyota

 

21. Influenza A virus neuraminidase inhibitors

Nongluk Sriwilaijaroen, Christopher J. Vavricka, Hiromasa Kiyota, and Yasuo Suzuki