Fasciola hepatica Methods and Protocols

This volume described basic and advanced protocols to study F. hepatica parasite biology. Chapters guide readers through protocols on different developmental stages of F. hepatica, characterize tegumental, secreted proteins, spatial and temporal gene expression, immunological effects of ESP on macrophages, eosinophil apoptosis, macrophages alternative activation, toll-like receptor interactions, obtain peritoneal and splenic dendritic cells of mice, and protocols to detect F. hepatica albendazole resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Fasciola hepatica: Methods and Protocols provides different topics in areas such a biochemistry, immunology, molecular biology, microscopy, vaccinology in order to extend the interest in this book to research community working with Fasciola hepatica and related trematodes.

1. Maintenance of Life Cycle at the Laboratory

Valeria Gayo, Martin Cancela, and Daniel Acosta

 

2. Microscopical Techniques to Analyse the Hepatic and Peritoneal Changes Caused by Fasciola hepatica Infection

M Teresa Ruiz-Campillo, Verónica Molina-Hernández, José Pérez, Isabel L Pacheco, Raúl Pérez, Alejandro Escamilla, Javier F Martínez-Moreno, Álvaro Martínez-Moreno, and Rafael Zafra

 

3. Isolation of Secreted and Tegumental Surface Proteins from Fasciola hepatica

Eduardo de la Torre-Escudero and Mark W. Robinson

 

4. Isolation and Analysis of Fasciola hepatica Extracellular Vesicles

Alicia Galiano, Maria Teresa Minguez, Christian M. Sánchez, and Antonio Marcilla

 

5. Cloning and Heterologous Expression of Protein-Coding Sequences in Escherichia coli

Martin Cancela and Gabriela Maggioli

 

6. Gene Silencing in the Liver Fluke Fasciola hepatica: RNA Interference

Gabriel Rinaldi, Nicolás Dell’Oca, Estela Castillo, and Jose F. Tort

 

7. Analysis of Gene Expression in Fasciola hepatica Juveniles and Adults by In Situ Hybridization

Estela Castillo and Uriel Koziol

 

8. Evasion of Host Immunity during Fasciola hepatica Infection

Robin J Flynn and Mayowa Musah-Eroje

 

9. Immunomodulatory Effect of Fasciola hepatica Excretory-secretory Products on Macrophages

Lorena Guasconi, Marianela Serradell, Diana T. Masih, and Laura S Chiapello

 

10. Study of Eosinophil Apoptosis Induced by Fasciola hepatica Excretory-secretory Products

Marianela C. Serradell, Lorena Guasconi, Laura A. Cervi, Laura S. Chiapello, and Diana T. Masih

 

11. Purification of native Fasciola hepatica Fatty Acid Binding Protein and the Induction of Alternative Activation of Human Macrophages

Olgary Figueroa-Santiago

 

12. Possible Role for Toll-Like Receptors in Interaction of Fasciola hepatica Excretory-Secretory Products with Human Monocyte Cell Line

Olgary Figueroa-Santiago

 

13. Evaluation of the Immune Regulatory Properties of Dendritic Cells during Fasciola hepatica Infection

Teresa Freire

14. Design of a Peptide–carrier Vaccine Based on the Highly Immunogenic Fasciola hepatica Leucine Aminopeptidase

Cecilia Salazar, José F Tort, and Carlos Carmona

 

15. Liver Fluke Vaccine Assessment in Cattle

Gabriela Maggioli, Cecilia Salazar, Federico Fossa, and Carlos Carmona

 

16. Testing Albendazole Resistance in Fasciola hepatica

Luis I. Alvarez, María Martinez Valladares, Candela Canton, Carlos E. Lanusse, and Laura Ceballos

 

17. Drug Targets: Screening for Small Molecules that Inhibit Fasciola hepatica Enzymes

Florencia Ferraro, Mauricio A. Cabrera, Guzmán I. Álvarez, and Ileana Corvo