Epstein Barr Virus Methods and Protocols

This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in the EBV field. Chapters in this book cover topics such as: viral life cycle, latency, EBV-associated diseases and EBV diagnostics; in vitro methods including organotypic cultures for the analysis of EBV-epithelial cell interactions; identification of the interacting viral and cellular proteins using affinity purification-mass spectrometry methods; 3D telomere FISH; transcription analysis using high-throughput RNA sequencing, qPCR and nuclear run-on assay; analysis of viral and cellular microRNAs; isolation and characterization of exosomes and the assessment of their function; characterization of the viral genome by terminal repeat analysis and sequencing; the use of chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for the analysis of Zta-DNA interactions; epigenetic analysis by bisulfite sequencing and ChIP; novel in vivo models for the study of EBV infection; and how immunological, virological, tissue culture and molecular methods can be combined to yield Good Manufacturing Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative diseases (PTLD). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Epstein Barr-Virus: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.

Foreword…
Preface…
Table of Contents…
Contributing Authors…

1. Current Trends and Alternative Scenarios in EBV Research
Janos Minarovits and Hans-Helmut Niller

2. Epstein-Barr Virus: Clinical Diagnostics
Hans-Helmut Niller and Georg Bauer

3. Establishment of EBV-Infected Lymphoblastoid Cell Lines
Noemi Nagy

4. Generation and Infection of Organotypic Cultures with Epstein-Barr Virus
Rachel M. Temple, Craig Meyers, and Clare E. Sample

5. Affinity Purification-Mass Spectroscopy Methods for Identifying Epstein-Barr Virus-Host Interactions
Anna A. Georges and Lori Frappier