Doubled Haploid Technology Volume 1: General Topics, Alliaceae, Cereals

This title offers 62 chapters divided among three volumes covering the latest topics dealing with Doubled Haploid (DH) technology, as well as methods to produce DHs in different species through different in vivo and in vitro approaches. Volume 1 explores general topics and transversal methods in DH technology; DH production in two alliaceae, onion, and leek; and DH production in cereals such as barley, durum, bread, oat, and japonica rice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Doubled Haploid Technology, Volume 1: General Topics, Alliaceae, Cereals is a valuable resource for scientists and researchers looking to learn more about this interesting and developing field.

Preface…
Table of Contents…
Contributing Authors…

Part I General Topics and Transversal Methods in DH Technology

1. Overview of In Vitro and In Vivo Doubled Haploid Technologies
Jose M. Seguí-Simarro, Nathanaël M.A. Jacquier, and Thomas Widiez

2. Applications of Doubled Haploids in Plant Breeding and Applied Research
Jens Weyen

3. Species with Haploid or Doubled Haploid Protocols
Jose M. Seguí-Simarro, Javier Belinchón Moreno, Marina Guillot Fernández, and Ricardo Mir Moreno

4. Analysis of Ploidy in Haploids and Doubled Haploids
Sergio J. Ochatt and Jose M. Seguí-Simarro

5. Methods for Chromosome Doubling
Mehran E. Shariatpanahi, Mohsen Niazian, and Behzad Ahmadi

Part II Doubled Haploids in Alliaceae

6. Doubled Haploid Onion (Allium cepa L.) Production via In Vitro Gynogenesis
Ali Ramazan Alan

7. In Vitro Gynogenesis in Leek (Allium ampeloprasum L.)
Fevziye Celebi-Toprak and Ali Ramazan Alan

Part III Doubled Haploids in Cereals

8. Barley Isolated Microspore Culture
Luís Cistué and Begoña Echávarri

9. Site-Directed Mutagenesis in Barley using RNA-GUided Cas Endonucleases during Microspore-Derived Generation of Doubled Haploids
Robert Eric Hoffie, Ingrid Otto, Hiroshi Hisano, and Jochen Kumlehn

10. Generation of Doubled Haploid Barley by Interspecific Pollination with Hordeum bulbosum
Pooja Satpathy, Sara Audije de la Fuente, Vivian Ott, Andrea Müller, Heike Büchner, Diaa Eldin S. Daghma, and Jochen Kumlehn

11. Bread Wheat Doubled Haploid Production by Anther Culture
Ana María Castillo, Isabel Valero-Rubira, Sandra Allué, Asunción Costar, and María Pilar Vallés

12. Unpollinated Ovaries Used to Produce Doubled Haploid Lines in Durum Wheat
Olfa Ayed Slama and Hajer Slim Amara

13. In Vitro Anther Culture for Doubled Haploid Plant Production in Spelt Wheat
Csaba Lantos and János Pauk

14. Production of Wheat Doubled Haploids through Intergeneric Hybridization with Maize
Pierre Devaux

15. Isolation of Staged and Viable Maize Microspores for DH Production
Philippe Vergne and Antoine Gaillard

16. Triticale Isolated Microspore Culture for Doubled Haploid Production
Priti Maheshwari and John D. Laurie

17. Oat (Avena sativa L.) Anther Culture
Marzena Warchol, Kinga Dziurka, Ilona Czyczylo-Mysza, Katarzyna Juzon, Izabela Marcinska, and Edyta Skrzypek

18. Oat Doubled Haploid Production through Wide Hybridization with Maize
Edyta Skrzypek, Marzena Warchol, Ilona Czyczylo-Mysza, Katarzyna Juzon, Kinga Dziurka, and Izabela Marcinska

19. Anther Culture and Chromosome Doubling in Mediterranean Japonica Rice
Isidre d’Hooghvorst, Irene Ferreres, and Salvador Nogués

20. High Throughtput Doubled Haploid Production for Indica Rice Breeding
Swapan K. Tripathy

Subject Index List…