Detection of Cell Death Mechanisms Methods and Protocols

This volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy and real-time multiplexed imaging assays.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and accessible, Detection of Cell Death Mechanisms: Methods and Protocols aims to ensure seamless execution of protocols on specific cell death type.

1. Determination of Caspase Activation by Western Blot

Hussein Chehade, Alexandra Fox, Gil Mor, and Ayesha B. Alvero

2. Detection of Unfolded Protein Response by Polymerase Chain Reaction

Alexander Paridon, Alexandra Fox, and Ayesha B. Alvero

3. Subcellular Fractionation to Demonstrate Activation of Intrinsic Apoptotic Pathway

Hussein Chehade, Alexandra Fox, Gil Mor, and Ayesha B. Alvero

4. A Triple-Parameter Based Laboratory Friendly Fluorescence Imaging to Identify Apoptosis in Live Cells

5. Flow Cytometric Analyses of p53-mediated Cell Cycle Arrest and Apoptosis in Cancer Cells

Nour N. Al Zouabi, Cai M. Roberts, Z. Ping Lin, and Elena S. Ratner

6. A Real-Time, Bioluminescent Apoptosis Assay

Kevin R. Kupcho and Andrew L. Niles

7. Detection of Anoikis Using Cell Viability Dye and Quantitation of Caspase Activity

8. Time and Dose Dependent Toxicity Studies in 3D Cultures Using a Luminescent Lactate Dehydrogenase Assay

Natasha Karassina, Peter Hofsteen, James Cali, and Jolanta Vidugiriene

9. Visualization and Quantification of Neutrophil Extracellular Traps

Mancy Tong and Vikki M Abrahams

10. 2In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps

11. Induction and Detection of Necroptotic Cell Death in Mammalian Cell Culture

Mikhail Chesnokov, Imran Khan, and Ilana Chefetz

12. Visualization of Necroptotic Cell Death through Transmission Electron Microscopy

Naresh Golla, Linda J. Hong, and Ilana Chefetz

13. Methodology for Comprehensive Detection of Pyroptosis

Yang Feng and Xiaoli Huang

14. Determination and Quantitation of Cytotoxic T Cell-Mediated Cell Death

Han-Hsuan Fu and Harry Qui

15. Detection of Immunogenic Cell Death in Tumor Vaccination Mouse Model

Kazuki Tatsuno, Patrick Han, Richard Edelson, and Douglas Hanlon

16. A Luminescent Assay to Quantify Cell Viability in Real Time

Peter Hofsteen, Natasha Karassina, James J. Cali, and Jolanta Vidugiriene

17. Kinetic Measurement of Apoptosis and Immune Cell Killing Using Live-Cell Imaging and Analysis

Jill E. Granger and Daniel M. Appledorn

Cai M Roberts and Elena S Ratner

19. Determining Cell Death Pathway and Regulation by Enrichment Analysis

Katherine Gurdziel

20. Transcription Factor Binding Site Identification and Enrichment Analysis

Joe L. Guy and Gil Mor