Cryopreservation and Freeze-Drying Protocols

This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Part I: Fundamental Aspects of Cryopreservation and Freeze-Drying

 

1. Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues

            Willem F. Wolkers and Harriëtte Oldenhof

 

2. Principles of Ice-Free Cryopreservation by Vitrification

            Gregory M. Fahy and Brian Wowk

 

3. The Principles of Freeze-Drying and Application of Analytical Technologies

            Kevin R. Ward and Paul Matejtschuk

 

4. Mathematical Modeling and Optimization of Cryopreservation in Single Cells

            James D. Benson

 

5. Mathematical Modeling of Protectant Transport in Tissues

            Ross M. Warner and Adam Z. Higgins

 

Part II: Technologies and Methods to Study Freezing and Drying

 

6. Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation

            Peter Kilbride and Julie Meneghel

 

7. Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of Biologics

            Shangping Wang, Susan Trammell, and Gloria D. Elliott

 

8. High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kinetics

            Jens O.M. Karlsson

 

9. Use of Ice Recrystallization Inhibition Assays to Screen for Compounds that Inhibit Ice Recrystallization

            Anna A. Ampaw, August Sibthorpe, and Robert N. Ben

 

10. DSC Analysis of Thermophysical Properties for Biomaterials and Formulations

            Wendell Q. Sun

 

11. Osmometric Measurements of Cryoprotective Agent Permeation into Tissues

            Kezhou Wu, Leila Laouar, Nadia Shardt, Janet A.W. Elliott, and Nadr M. Jomha

 

12. Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes

            Ariadna Corral, Alberto Olmo, and Ramón Risco

 

13. Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological Research

            Willem F. Wolkers and Harriëtte Oldenhof

 

14. Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements

            Guanglin Yu, Rui Li, and Allison Hubel

 

Part III: Cryopreservation and Freeze-Drying Protocols

 

15. Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm

            Harriëtte Oldenhof, Willem F. Wolkers, and Harald Sieme

 

16. Cryopreservation of Avian Semen

            Henri Woelders

 

17. Cryopreservation of Mouse Sperm for Genome Banking

            Yuksel Agca and Cansu Agca

 

18. Cryopreservation of Marine Invertebrates: From Sperm to Complex Larval Stages

            Estefania Paredes, Pablo Heres, Catarina Anjos, and Elsa Cabrita

 

19. Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into a Cooling Agent

            Mengying Wang, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, and Vladimir Isachenko

 

20. Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification as Successful Methods for Cryogenic Storage

            Victoria Keros and Barry J. Fuller

 

21. Vitrification of Porcine Oocytes and Zygotes in Microdrops on a Solid Metal Surface or Liquid Nitrogen

            Tamas Somfai and Kazuhiro Kikuchi

 

22. Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research

            Yuksel Agca and Cansu Agca

 

23. Cryopreservation and Thawing of Human Ovarian Cortex Tissue Slices

            Jana Liebenthron and Markus Montag

 

24. Vitrification: A Simple and Successful Method for Cryostorage of Human Blastocysts

            Juergen Liebermann

 

25. Vitrification of Equine In Vivo-Derived Embryos, after Blastocoel Aspiration

            Carolina Herrera

 

26. Frozen Blood Reserves

            Johan W. Lagerberg

 

27. Isolation, Cryopreservation, and Characterization of iPSC-Derived Megakaryocytes

            Denys Pogozhykh, Rainer Blasczyk, and Constança Figueiredo

 

28. Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem Cells

            Melany López and Ali Eroglu

 

29. Chemically-Defined and Xeno-Free Cryopreservation of Human Induced Pluripotent Stem Cells

            Juliette Seremak and Ali Eroglu

 

30. Protocol for Cryopreservation of Endothelial Monolayers

            Leah A. Marquez-Curtis, Nasim Eskandari, Locksley E. McGann, and Janet A.W. Elliott

 

31. Vitrification of Heart Valve Tissues

            Kelvin G.M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene, and Lia H. Campbell

 

32. Cryopreservation of Algae

            Estefania Paredes, Angela Ward, Ian Probert, Léna Gouhier, and Christine N. Campbell

 

33. Cryopreservation of Fern Spores and Pollen

            Anna Nebot, Victoria J. Philpott, Anna Pajdo, and Daniel Ballesteros

 

34. Cryopreservation of Plant Cell Lines Using Alginate Encapsulation

            Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack

 

35. Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrificati