Computer-Aided Tissue Engineering Methods and Protocols

This volume details protocols encompassing different aspects of computer aided design and manufacturing of 3D scaffolds and biofabricated constructs for tissue engineering applications. Chapters are divided into four parts covering optimization of scaffold architectures for computer aided tissue engineering, synthetic routes to biomaterials compatible, technological platforms and manufacturing processes, and relevant applicative scenarios. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Computer-Aided Tissue Engineering: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.

1. Biomimetic Boundary-based Scaffold Design for Tissue Engineering Applications

Henrique A. Almeida and Paulo J. Bártolo

2. Triply Periodic Minimal Surfaces (TPMS) for the Generation of Porous Architectures Using Stereolithography

Sebastien B.G. Blanquer

3. 3D Printing Of Functionally-graded Films by Controlling Process Parameters

A. Bonfanti, L. Domenicale, and A. Bhaskar

4. Photocurable Biopolymers for Coaxial Bioprinting

Marco Costantini, Andrea Barbetta, Wojciech Swieszkowski, Dror Seliktar, Cesare Gargioli_,  and Alberto Rainer

5. Synthesis of an UV-curable Divinyl-fumarate Poly-e-Caprolactone for Stereolithography Applications

Ronca A., Ronca S., Forte G., and Ambrosio L.

6. Nanocomposite Clay-based Bioinks for Skeletal Tissue Engineering

Gianluca Cidonio, Michael Glinka, Yang-Hee Kim, Jonathan I. Dawson, and Richard O.C. Oreffo

7. Additive Manufacturing using Melt Extruded Thermoplastics for Tissue Engineering

Andrea Roberto Calore, Ravi Sinha, Jules Harings, Katrien V. Bernaerts, Carlos Mota, and Lorenzo Moroni

8. Computer-aided Wet-spinning

Dario Puppi and  Federica Chiellini

9. Production of Scaffolds Using Melt Electrospinning Writing and Cell Seeding

E. C. Bolle, D. Nicdao, P.D. Dalton, and T.R. Dargaville

10. Low Voltage Continuous Electrospinning: A Versatile Protocol for Patterning Nano-and Micro-Scared Fibers for Cell Interface

Zhaoying Li, Xia Li, and Yan Yan Shery Huang

11. Direct Write Deposition of Thermogels

Sara Maria Giannitelli, Valeria Chiono, and Pamela Mozetic

12. 3D Bioprinting of Complex, Cell-laden Alginate Constructs

Atabak Ghanizadeh Tabriz, Dirk-Jan Cornelissen, and Wenmiao Shu

13. Surface Tension-assisted Additive Manufacturing of Tubular, Multicomponent Biomaterials

Elia A. Guzzi, Héloïse Ragelle, and Mark W. Tibbitt

14. Bioprinting of Complex Vascularized Tissues

Wei Zhu, Claire Yu, Bingjie Sun, and Shaochen Chen

15. A Scaffold-free 3D Bioprinted Cartilage Model for in vitro Toxicology           

Pallab Datta, Yang Wu, Yin Yu, Kazim K. Moncal, and Ibrahim T. Ozbolat