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Clinical Applications of Mass Spectrometry in Biomolecular Analysis Methods and Protocols




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Genere:Libro
Lingua: Inglese
Editore:

Humana

Pubblicazione: 09/2022
Edizione: 2nd ed. 2022





Trama

This updated volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and limited need for specialized reagents. These techniques are essential in laboratory fields including endocrinology, biochemical genetics, drug analysis, proteomics, and pathogen identification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols, Second Edition is an ideal resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories.




Sommario

1. Introduction to Mass Spectrometry for Bimolecular Analysis in a Clinical Laboratory

            Y. Victoria Zhang and Uttam Garg

 

2. System Performance Monitoring in Clinical Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

            Shannon Haymond

 

3. Tandem Mass Spectrometry for the Analysis of Plasma/Serum Acylcarnitines for the Diagnosis of Certain Organic Acidurias and Fatty Acid Oxidation Disorders

            David Scott, C. Clinton Frazee III, Bryce Heese, and Uttam Garg

 

4. Quantification of Plasma S-Adenosylmethionine and S-Adenosylhomocysteine Using Liquid Chromatography Electrospray Tandem Mass Spectrometry

            Erland Arning, Brandi Wasek, and Teodoro Bottiglieri

 

5. Quantitation of Aldosterone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

            J. Grace Van Der Gugten and Daniel T. Holmes

 

6. Comprehensive Determination of Amino Acids for Diagnosis of Inborn Errors of Metabolism

            Stephen M. Roper, Annette L. Weindel, and Dennis J. Dietzen

 

7. Quantification of Branched-Chain Amino Acids in Plasma by High-Performance Liquid Chromatography-Tandem Mass Spectrometry (LC- MS/MS)

            Hamed Piri-Moghadam, Alan Miller, Debra Pronger, Faye Vicente, Joel Charrow, Shannon Haymond, and David C. Lin

 

8. Quantitation of Butyrylcarnitine, Isobutyrylcarnitine, and Glutarylcarnitine in Urine Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS)

            Judith A. Hobert, Stephen A. Brose, and Marzia Pasquali

 

9. Quantification of Free and Total Carnitine in Serum Using Liquid Chromatography Tandem Mass Spectrometry

            Serena Baird, C. Clint Frazee III, and Uttam Garg

 

10. Sensitive and Robust LC-MS/MS Analysis of Salivary Cortisol in Negative Mode

            Wayne B. Anderson, Putuma P. Gqamana, and Y. Victoria Zhang

 

11. Measurement of Urine Free Cortisol and Cortisone by LC-MS/MS

            Julie A. Ray, Erik Kish-Trier, and Lisa M. Johnson

 

12. Laboratory Diagnosis of Cerebral Creatine Deficiency Syndromes by Determining Creatine and Guanidinoacetate in Plasma and Urine

            Ning Liu and Qin Sun

 

13. Quantitation of Estradiol and Testosterone in Serum Using LC-MS/MS

            Ryan C. Schofield, Daniel Kirchoff, and Dean C. Carlow

 

14. Quantitation of Fatty Acids in Serum/Plasma and Red Blood Cells by Gas Chromatography-Negative Chemical Ionization-Mass Spectrometry

            Erik Kish-Trier and Tatiana Yuzyuk

 

15. Quantitation of ?-Aminobutyric Acid in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry

            Erland Arning, Brandi Wasek, and Teodoro Bottiglieri

 

16. A Simple, Fast, and Reliable LC-MS/MS Method for the Measurement of Homovanillic Acid and Vanillylmandelic Acid in Urine Specimens

            Vrajesh Pandya and Elizabeth L. Frank

 

17. Quantitation of Neuroblastoma Markers Homovanillic Acid (HVA) and Vanillylmandelic Acid (VMA) in Urine by Gas Chromatography-Mass Spectrometry (GC/MS)

            Melissa Beals, Bheemraj Ramoo, C. Clinton Frazee III, and Uttam Garg

 

18. Quantification of 5-Hydroxyindoleacetic Acid in Urine by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry

            Heather A. Nelson and Elizabeth L. Frank

 

19. Quantitation of IgG Subclasses in Serum Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

            J. Grace Van Der Gugten, Daniel T. Holmes, and Andre Mattman

 

20. Quantification of Insulin Analogs by Liquid Chromatography-High Resolution Mass Spectrometry

            Erica M. Fatica, Nicholas E. Larkey, and Ravinder J. Singh

 

21. Differentiation of Common IGF-1 Variants Using HRAM-MS COM Determination with Follow-Up MS/MS Verification

            Nicholas E. Larkey, Erica M. Fatica, and Ravinder J. Singh

 

22. Monitoring and Identifying Insulin-Like Growth Factor 1 Variants by Liquid Chromatography-High Resolution Mass Spectrometry in a Clinical Laboratory

            Ievgen Motorykin, Allison Li, and Zengru Wu

 

23. Quantitation of Lactate in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry

            Brandi Wasek and Erland Arning

 

24. Multiplex Lysosomal Enzyme Activity Assay on Dried Blood Spots Using Tandem Mass Spectrometry

            Hsuan-Chieh (Joyce) Liao and Hsiao-Jan Chen

 

25. Plasma Lysosphingolipid Biomarker Measurement by Liquid Chromatography-Tandem Mass Spectrometry

            Brandon B. Stauffer and Chunli Yu

 

26. Detection of 13C-Mannitol and Other Saccharides Using Tandem Mass Spectrometry for Evaluation of Intestinal Permeability, or Leaky Gut

            Nicholas E. Larkey, Erica M. Fatica, and Ravinder J. Singh

 

27. LC-MS/MS Method for High Throughput Analysis of Methylmalonic Acid in Serum, Plasma, and Urine: Method for Analyzing Isomers without Chromatographic Separation

            Mark M. Kushnir, Gordon J. Nelson, Elizabeth L. Frank, and Alan L. Rockwood

 

28. Quantitation of 5-Methyltetrahydrofolate in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry

            Erland Arning, Brandi Wasek, and Teodoro Bottiglieri

 

29. Screening of Organic Acidurias by Gas Chromatography Mass Spectrometry (GC-MS)

            David Scott, C. Clinton Frazee III, and Uttam Garg

 

30. Identification of Urine Organic Acids for the Detection of Inborn Errors of Metabolism Usin











Altre Informazioni

ISBN:

9781071625644

Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 254 x 178 mm Ø 1252 gr
Formato: Copertina rigida
Illustration Notes:XVI, 547 p. 88 illus., 55 illus. in color. With online files/update.
Pagine Arabe: 547
Pagine Romane: xvi


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