Part I Culturing and morphology
1. Rat pheochromocytoma PC12 cells in culture
Chin-Tien Wang
2. Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation
Alejandra Boronat-Garcia, Marcela Palomero-Rivero, Magdalena Guerra-Crespo
3. Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells
Keita Harada, Hidetada Matsuoka, Masumi Inoue
4. Transmission electron microscopy: a method for studying the adrenochromaffin cells
Anna Capaldo, Maria De Falco, Luigi Rosati and Vincenza Laforgia
5. Immunogold for protein location in chromaffin cells
Rafael Luján, Rocio Alfaro-Ruiz and Carolina Aguado
6. Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy
Chung Yu Chan, Sue Han, Xin Wang, Xiaoli Guo and Ling-Gang Wu
Part II Stimulus-secretion coupling
7. Online detection of catecholamine release from the perfused rat adrenal gland
Ricardo Borges, Ana de la Iglesia and Antonio G. García
8. On-line recording of perifused chromaffin cells
Ricardo De Pascual and Luis Gandía
9. Recording of chromaffin cell electrical activity in situ in acute adrenal slices
Nathali Guerineau
10. Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats
Tzitzitlini Alejandre-García, Pedro Segura-Chama, Oscar Javier Parada-Parra, Diana Millán-Aldaco and Arturo Hernández-Cruz.
11. Measurements of calcium in chromaffin cell organelles using targeted aequorins.
Jaime Santo-Domingo, Pilar Álvarez-Illera, Pablo Montenegro, Rosalba I Fonteriz, Mayte Montero and Javier Álvarez
12. Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements
Muhmmad Omar-Hmeadi, Liangwen Liu, Santiago Echeverry, and Sebastian Barg
13. Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell
Elizabeth P. Seward and Robert C. Wykes
14. Quantal release analysis of electrochemically active molecules using single-cell amperometry
Natalia Domínguez, Pablo Montenegro and J. David Machado
15. Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs
Giulia Tomagra, Claudio Franchino, Emilio Carbone, Andrea Marcantoni, Alberto Pasquarelli, Federico Picollo, Valentina Carabelli
16. Vesicle collision protocols for the study of quantum size and exocytotic fraction released
Soodabeh Majdi, Alex S. Lima and Andrew Ewing
17. Patch Amperometry and Intracellular Patch Electrochemistry
Eugene V. Mosharov and Manfred Lindau
18. Artificial cells for dissecting exocytosis
Yuanmo Wang and Ann-Sofie Cans
Part III Chromaffin granules and membrane traffic
19. Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells
Arlek Gonzalez-Jamett, María Constanza Maldifassi, Ana María Cárdenas
20. Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells
José Villanueva, Yolanda Gimenez-Molina and Luis M. Gutiérrez
21. Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging
Adekunle T. Bademosi and Frédéric A. Meunier
Part IV Miscellanea
22. Determination of catecholamines in a small volume (25µl) of plasma from conscious mouse tail vein
Kechun Tang and Sushil K. Mahata
23. Quantification of chromogranin A and its fragments in biological fluids
Flavio Curnis, Barbara Colombo and Angelo Corti
24. Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds
María F. Cano Abad and Manuela G. López
