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1. Protein-Metabolite Interactions Shape Cellular Metabolism and Physiology
Prathibha Muralidhara and Jennifer C. Ewald
2. Affinity Purification Protocol Starting with a Small Molecule as Bait
Nicolas E. Figueroa, Itzell E. Hernandez-Sanchez, Israel Maruri-Lopez, and Monika Chodasiewicz
3. Cellular Thermal Shift Assay for the Detection of Small Molecule-Target Interactions in Arabidopsis Cells
Qing Lu and Eugenia Russinova
4. Solvent-Induced Protein Precipitation for Drug Target Discovery
Xiaolei Zhang, Lianghai Hu, and Mingliang Ye
5. Identification of Plant Protein-Metabolite Interactions by Limited Proteolysis-Coupled Mass Spectrometry (LiP-MS)
Jhon Venegas-Molina, Petra Van Damme, and Alain Goossens
6. Limited Proteolysis-Mass Spectrometry to Identify Metabolite-Protein Interactions
Aleš Holfeld, Jan-Philipp Quast, Roland Bruderer, Lukas Reiter, Natalie de Souza, and Paola Picotti
7. Proteome Integral Solubility Alteration (PISA) for High-Throughput Ligand Target Deconvolution with Increased Statistical Significance and Reduced Sample Amount
Massimiliano Gaetani and Roman A. Zubarev
8. Parallel Analysis of Protein–Protein and Protein–Metabolite Complexes Using a Single-Step Affinity Purification
Marcin Luzarowski and Aleksandra Skirycz
9. Detecting and Characterizing Interactions of Metabolites with Proteins by Saturation Transfer Difference Nuclear Magnetic Resonance (STD-NMR) Spectroscopy
Ruslan Nedielkov and Heiko M. Möller
10. PROMIS: Co-Fractionation Mass Spectrometry for Analysis of Protein-Metabolite Interactions
Marcin Luzarowski, Ewelina M. Sokolowska, Dennis Schlossarek, and Aleksandra Skirycz
11. Metabolomics: Going Deeper, Going Broader, Going Further
Sofia Moco and Joerg M. Buescher
12. Specifics of Metabolite-Protein Interactions and Their Computational Analysis and Prediction
Dirk Walther
13. Biophysical Approaches for the Characterization of Protein-Metabolite Interactions
Anja Thalhammer and Nina K. Bröker
14. Databases and Tools to Investigate Protein-Metabolite Interactions
Leonardo Perez de Souza and Alisdair R. Fernie
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