Cell Cycle Checkpoints Methods and Protocols

Determination of CHK1 Cellular Localization by Immunofluorescence Microscopy.- Detection of Post-Translationally Modified p53 by Western Blotting.- Global Analyses to Identify Direct Transcriptional Targets of p53.- Analysis of Replication Dynamics Using the Single-Molecule DNA Fiber Spreading Assay.- Measuring Translation Efficiency by RNA Immunoprecipitation of Translation Initiation Factors.- DNA Affinity Purification: A Pulldown Assay for Identifying and Analyzing Proteins Binding to Nucleic Acids.- A Novel Strategy to Track Lysine-48 Ubiquitination by Fluorescence Resonance Energy Transfer.- The DNA Damage Response in Xenopus laevis Cell-Free Extracts.- Mammalian Cell Fusion Assays for the Study of Cell Cycle Progression by Functional Complementation.- Knockdown of Target Genes by siRNA In Vitro.- Assaying Cell Cycle Status Using Flow Cytometry.- Using TUNEL Assay to Quantitate p53-Induced Apoptosis in Mouse Tissues.- Generation and Analysis of dsDNA Breaks for Checkpoint and Repair Studies in Fission Yeast.- Calreticulin Exposure in Mitotic Catastrophe.- Quantification of eIF2a Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy.- Clonogenic Assays to Detect Cell Fate in Mitotic Catastrophe.