Cardiovascular Development Methods and Protocols

This second edition brings together a variety of method used in the field of cardiovascular development research. Chapters guide readers through protocols on lymphatic development, lymphatic staining techniques, lymphatic endothelial, tissue clearing techniques, and muscle cell isolation and culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls

Authoritative and practical, Cardiovascular Development: Methods and Protocols, Second Edition seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research.

1. Tissue clearing and 3-D visualization of Vasculature with the PEGASOS Method

Dian Jing, Yi Men, and Hu Zhao

2. Preparation and Identification of Cardiac Myofibrils from Whole Heart Sample

Heidi Creed and Carl W. Tong

3. RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells

Hongjiang Si and Xu Peng

4. Preparation of Neonatal Rat Papillary Muscles for Contractile Studies

Steven Jokerst, Damir Nizmutdinov, Charley Edgar, April M. Kaspick, Carl W. Tong, and David E. Dostal

5. In Vitro Study of permeability in Lymphatic Endothelial Cells Responding to Histamine

Hongjiang Si

6. Optimizing the Differentiation of Cardiomyocytes from Human Induced Pluripotent Derived-Stem Cells

Melanie Gartz and Jennifer L. Strande

7. Analysis of Lymphatic Vessel Formation by Whole-mount Immunofluorescence Staining

Jian Wang, Yuwei Dong, Mariappan Muthuchamy, David C. Zawieja, and Xu Peng

8. Isolation and Culture of Mouse Lymphatic Endothelial Cells from Lung Tissue

Philip E. Lapinski and Philip D. King

9. Laser-induced Choroidal Neovascularization in Rats      

Min Zhao, Wankun Xie, Travis W. Hein, Lih Kuo, and Robert H. Rosa, Jr.

10. Visualizing Three-Dimensional Vascular Networks in the Mouse Embryonic Hindbrain

Chenshen Huang, Liangjing Wu, Jian Wang, Binu Tharakan, and Xu Peng

11. Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy

Samantha J. Fredrickson, Tanner G. Hoog, and Ryan S. Udan

Tianhao Zhou and Jian Wang

13. Visualization of Fundus Blood Vessels

Wankun Xie, Min Zhao, Travis W. Hein, Lih Kuo, and Robert H. Rosa, Jr.

14. Transient Transgenics: An Efficient Method to Identify Gene Regulatory Elements

15. Isolation of Lymphatic Muscle Cells (LMCs) from Rat Mesentery

16. Isolation of Adult Mouse Cardiomyocytes using Langendorff Perfusion Apparatus

17. Visualization of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining

Jian Wang, Yuwei Dong, Yu Xi, and Xu Peng