Cardiac Cell and Gene Transfer

Heart disease is the leading cause of death in developed countries. Recent experimental advances featuring cellular, molecular, and genetic tools and technologies offer the potential for new therapeutic strategies directed toward remediation of inherited and acquired heart diseases. Whether these recent basic science advances will ultimately translate to clinical efficacy for patients with heart disease is unknown and is important to ascertain. Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications is designed to provide the reader with up-to-date coverage of a myriad of specific methodo- gies and protocols for gene and cell transfer to the myocardium. Each chapter features a “Notes” section that provides useful “how to” problem-solving insights that are often left unstated in standard published protocols. Cardiac Cell and Gene Transfer: Principles, Protocols, and Appli- tions addresses principles and applications of cell and gene transfer to the heart, including protocols for vector production and purification. Detailed step-by-step methods and applications for first/second-generation adenoviral vectors, adeno-associated vectors, gutted adenoviral vectors, and lentiviral vectors are included. Additionally, detailed methods for cardiac cell grafting and transplantation are provided, and these chapters highlight the prospects of cell-based therapies for cardiac repair. The book also covers specific in vivo techniques for cardiac gene transfer, and specifies subsequent cellular and organ-level physiological assessment techniques and protocols. Accordingly, this book is designed for basic science and clinical researchers in the academic, pharmaceutical, and biotechnology sectors of the cardiovascular community.

Vectors for Cardiac Gene Transfer.- Adenoviral Vectors.- Gutted Adenoviral Vectors for Gene Transfer to Muscle.- Dual Vector Expansion of the Recombinant AAV Packaging Capacity.- Lentivirus Vector-Mediated Gene Transfer to Cardiomyocytes.- Cell Transfer to Myocardium.- Cell Therapy in the Heart.- Cardiac Cell Transplantation.- Cell Grafting for Cardiac Repair.- Vectors for Cardiac Gene Transfer.- Suppressor tRNAs.- Antisense Oligonucleotides.- Theoretical and Technical Considerations for Gene Transfer into Vascularized Cardiac Transplants.- Isolation, Culture, and Gene Transfer of Adult Canine Cardiac Myocytes.- Myofilament Protein Phosphorylation by PKC in Genetically Engineered Adult Cardiac Myocytes.- In Vivo Cardiac Gene Transfer.- Embryonic and Neonatal Cardiac Gene Transfer In Vivo.- Efficient Viral Gene Transfer to Rodent Hearts In Vivo.- Direct Gene Transfer to the Adult Rodent Myocardium In Vivo.- Adenovirus-Mediated Gene Transfer to Cardiac Myocytes In Vivo Using Catheter-Based Procedures.- Coronary Perfusion Cocktails for In Vivo Gene Transfer.- Modification of In Vivo Cardiac Performance by Intracoronary Gene Transfer of ?-Adrenergic Receptor Signaling Components.- Protocols for Hemodynamic Assessment of Transgenic Mice In Vivo.