Brain Organoid Research

This volume explores multiple methods and approaches used to generate human brain and neuroretinal organoids to address fundamental questions in human brain research. The chapters in this book cover topics such as self-organizing brain organoids with ventricles amenable to injection and electroporation; visualization of 3D organoids though the latest advancements in microscopy; generation of 3D retinal tissue with physiologically competent, light-sensitive photoreceptors; modeling brain tumors using genetically edited brain organoids; and brain organoids as a model to study Zika virus and SARS-CoV-2 infections. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. 

 

Comprehensive and cutting-edge, Brain Organoid Research is a valuable resource for researchers at various levels of learning, ranging from undergraduate students, early researchers, and advanced laboratories. This book aims to be instrumental in moving this developing field forward.

Series Preface…

Preface…

Table of Contents…

Contributing Authors…

1. Self-Organizing Brain Organoids with Ventricles Amenable to Injection and Electroporation

Stefano L. Giandomenico and Madeline A. Lancaster

2. Profiling Cell Type-Specific Gene Regulatory Regions in Human Cortical Organoids

Theresa M. Schütze, Nora Bölicke, Katrin Sameith, and Mareike Albert

3. Visualization of 3D Organoids Through the Latest Advancements in Microscopy

Manoj Kumar, Riya Rauthan, Debojyoti Chakraborty, and Senthil Arumugam

4. Generation and Isolation of Retinal Ganglion Cells and Photoreceptors from Human iPSC-Derived Retinal Organoids by Magnetic-Activated Cell Sorting

Gael Orieux, Oriane Rabesandratana, Giuliana Gagliardi, and Olivier Goureau

5. Micromanipulator-Assisted Subretinal Transplantation of Human Photoreceptor Reporter Cell Suspensions into Mice

6. Generation of Three-Dimensional Retinal Tissue with Physiologically Competent, Light Sensitive Photoreceptors from Human Induced Pluripotent Stem Cells

Silvia Aparicio-Domingo, Miguel Flores-Bellver, Hannah Cobb, Kang Li, Bogi Conrad, Conan Chen, Joseph A. Brzezinski IV, and M. Valeria Canto-Soler

7. Quantitative Assessment of Fluorescent Reporter Expression in 3D Retinal Organoids

Anne Vielle, Nathan Mathiyakom, Helen Li, Anna C. Howell, and M. Natalia Vergara

8. A Three-Dimensional Organoid Culture System to Model Invasive Patterns of Patient-Derived Glioma Stem Cells

Aruljothi Mariappan, Theda Knauth, Roberto Pallini, and Jay Gopalakrishnan

9. Modeling Brain Tumors using Genetically Edited Brain Organoids 

Linbo Li and Shan Bian

10. Brain Organoids as a Model to Study Zika Virus and SARS-CoV-2 Infections

Veronica Krenn, Luiza M. Higa, and Patricia P. Garcez

Philipp Niklas Ostermann, Anand Ramani, Ann Kathrin Bergmann, Ortwin Adams, Jay Gopalakrishnan, and Heiner Schaal

Subject Index List…