Bioluminescence Methods and Protocols, Volume 1

Gene Cloning and Functional Analysis of the Luciferase from Luminous Syllids of the Genus Odontosyllis.- Synthetic Coelenterazine Derivatives and Their Application for Bioluminescence Imaging.- Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging.- Biosynthesis-Inspired Deracemizative Production of D-Luciferin In Vitro by Combining Luciferase and Thioesterase.- Production of Metridia Luciferase in Native Form by Oxidative Refolding from E. coli Inclusion Bodies.- Production of Copepod Luciferases via Baculovirus Expression System.- Molecular Tension Probe for In Vitro Bioassays.- Optimized Loop-Mediated Amplification (LAMP) Allows Single Copy Detection Using Bioluminescent Assay in Real Time (BART).- A Simple and Rapid Bioluminescence-Based Functional Assay of Organic Anion Transporter 1 as a d-Luciferin Transporter.- A Simple Bioluminescent Assay for the Screening of Cytotoxic Molecules against the Intracellular Form of Leishmania infantum.- A Simple, Robust, and Affordable Bioluminescent Assay for Drug Screening against Infective African Trypanosomes.- Imaging of Autonomous Bioluminescence Emission from Single Mammalian Cells.- Rapid Single-Cell Detection of Beer-contaminating Lactic Acid Bacteria Using Bioluminescence/Rapid Microbe Detection.- Bioluminescence of Aliivibrio fischeri in Artificial Seawater and Its Application in Fungicide Sensing.- A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor.- Design of an Intron-Retained Bioluminescence Reporter and Its Application in Imaging of Pre-mRNA Splicing in Living Subjects.- Generation of Bi-Reporter Expressing Tri-Segmented Arenavirus.- Bioluminescent and Fluorescent Reporter-Expressing Recombinant SARS-CoV-2.- Generation, Characterization, and Applications of Influenza A Reporter Viruses.- Optimized Aequorin Reconstitution Protocol to Visualize Calcium Ion Transients in the Heart of TransgenicZebrafish Embryos In Vivo.- Quantification and Imaging of Exosomes via Luciferase-Fused Exosome Marker Proteins: ExoLuc System.- Bioluminescent Tracking of Human Induced Pluripotent Stem Cells In Vitro and In Vivo.- Noninvasive In Vivo Tracking of Mammalian Cells Stably Expressing Firefly Luciferase.- Bioluminescence Imaging for Evaluation of Antitumor Effect In Vitro and In Vivo in Mice Xenografted Tumor Models.- Detection of Spontaneous Bone Metastases of Solid Human Tumor Xenografts in Mice.- In Vivo Imaging Analysis of an Inner Ear Drug Delivery in Mice: Comparison of Inner Ear Drug Concentrations Over Time.- Protocols for the Evaluation of a Lymphatic Drug Delivery System Combined with Bioluminescence to Treat Metastatic Lymph Nodes.- In Vivo Bioluminescent Imaging of Rabies Virus Infection and Evaluation of Antiviral Drug.- Imaging Infection by Vector-Borne Protozoan Parasites Using Whole-Mouse Bioluminescence.- Longitudinal Tracing of Lyssavirus Infection in Mice via InVivo Bioluminescence Imaging.- Dual-Luciferase-Based Fast and Sensitive Detection of Malaria Hypnozoites for the Discovery of Anti-Relapse Compounds.- Synthetic Assembly DNA Cloning of Multiplex Hextuple Luciferase Reporter Plasmids.- Multiplex Hextuple Luciferase Assaying.- Molecular Imaging of Tumor Progression and Angiogenesis by Dual Bioluminescence.