Biocatalyst Immobilization Foundations and Applications

Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes.

Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends.

CHAPTER 1 The enzyme, the support, and the immobilization strategy: The key findings to a desirable biocatalyst

Karen Rodriguez-Nunez, Claudia Bernal, and Juan M. Boli´var

CHAPTER 2 Selection and modification of enzymes prior to immobilization

Ja Hyun Lee, Hah Young Yoo, and Seung Wook Kim

CHAPTER 3 Various approaches to enzyme immobilization

Syed Awais Attique, Qurat ul ain, Nazim Hussain, Muhammad Bilal, and Hafiz M.N. Iqbal

CHAPTER 4 Postimmobilization treatments before applications

Karolina Labus and Jolanta Bryjak

CHAPTER 5 Support-free immobilization

Roberta Bussons Rodrigues Val

CHAPTER 6 Measuring and reporting enzyme´s immobilization efficiency

Paula Nicolas, Carla Jos

CHAPTER 7 Some recent innovations related to enzyme immobilization

Bhaskar Das, Satyam, and Sanjukta Patra

CHAPTER 8 Enzyme immobilization for use in nonconventional media

Maria Laura Foresti and Maria Lujan Ferreira

CHAPTER 9 Assaying the biological activities of immobilized enzymes

Daniel Sanchez and Maria Lujan Ferreira

CHAPTER 10 Enzyme immobilization on nanomaterials and nanostructured supports

Azeem Intisar, Mateen Haider, Muhammad Imran Din, Nazim Hussain, Muhammad Bilal, and Hafiz M.N. Iqbal

CHAPTER 11 Oriented immobilization of biomolecules on small surfaces

Masumi Iijima and Shunichi Kuroda

CHAPTER 12 Affinity immobilization and affinity layers

Meryam Sardar and Kainat Mirza

CHAPTER 13 New enzymatic reactor designs: From enzymatic batch to 3D microreactors and monoliths

Kim Shortall, Katarzyna Szymanska, Cristina Carucci, Tewfik Soulimane, and Edmond Magner

CHAPTER 14 Immobilization of multienzymes: Problems and solutions

Yingjie Du, Le Zhong, Ziyuan Wang, Yuxiao Feng, Jiandong Cui, and Shiru Jia

CHAPTER 15 An introduction to enzymatic biosensors

Tayyaba Anjum, Nazim Hussain, Muhammad Bilal, and Hafiz M.N. Iqbal

CHAPTER 16 Industrial applications of immobilized enzymes: Food and beyond

Maria Lujan Ferreira

CHAPTER 17 Future perspectives in enzyme immobilization

Lorena Wilson, Andr

PhD in Chemistry María Luján Ferreira is a Principal Investigator at CONICET (NATIONAL COUNCIL OF SCIENTIFIC AND TECHNICAL RESEARCH) of Argentina. She has published more than 150 articles in peer reviewed, international journals, and has participated with presentations in more than 80 argentinian scientific events and more than 120 international scientific meetings. Dr. Ferreira has also directed three Assistant Investigators at CONICET.
Her research focuses on all practical aspects of enzyme immobilization (including applied biotechnology) and the details of the experimental design of enzymatic reactions: how to prepare immobilized biocatalysts and sampling in biocatalytic reactions ( from biphasic and multiphasic reaction media) avoiding mistakes and optimizing procedures. Her interests include the molecular modeling of biocatalysts. In more than 20 years of scientific career she has studied polymerization catalysts, heterogeneous metallic catalysts and recently also nanozymes and ionzymes applied in environmental remediation (of dyes and pharmaceuticals) using advanced oxidation processes.