Questo prodotto usufruisce delle SPEDIZIONI GRATIS
selezionando l'opzione Corriere Veloce in fase di ordine.
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1. Overview
2. Cell Culture Assays
2.1 Cell Cycle Assay: Propidium Iodide Marking
2.2 Apoptosis Assay: Annexin V/Propidium Iodide Marking
2.3 Cytotoxicity in Caco-2 cells by the Alamar blue method
2.4 Viability in Caco-2 cells stimulated with lipopolysaccharide (LPS)
2.5 Determination of Nitric oxide (NO) by in vitro experiment
2.6 Analysis of specific cytotoxicity against RAW 264.7 for determination anti-inflammatory activity in vitro
2.7 Scratching of Fibroblast cells (scratch assay)
2.8 Evaluation of the in vitro inflammatory response in AGS cells
3. Phytochemical Analysis
3.1 Preliminary phytochemical assays
3.2 Quantitative analysis of secondary metabolites
3.3 Thin Layer Chromatography Analysis
3.4 Analysis by High Performance Liquid Chromatography (HPLC)
3.5 Mass spectrometry
3.6 Electrospray Ionization Mass Spectrometry (ESI-MS) analysis3.7 Identification of phenolic compounds by HPLC-LC-ESI- MS/MS
3.8 Quantitative determination of total alkaloids content by HPLC3.9 Quantitative determination of total alkaloids content by HPLC
4. Preclinical Drug Dose Calculation
4.1 Conversion based on normalization of dose-to body surface area
4.2 Determination of animal equivalent dose5. Toxicology Studies (In Vitro and In Vivo)
5.1 Cytotoxicity evaluation using Alamar blue5.2 Micronucleus test
5.3 Comet test5.4 Effect on cell cycle
5.5 In vivo acute toxicity study
5.6 Subchronic toxicity (repeated doses)
6. Standard Procedure for Anesthesia in Preclinical Experiments
6.1 Planning and anesthetic care
6.2 Pre-Procedure
6.3 Experimental Procedure
7. General Considerations and Collection of Animal Blood
7.1 Vein blood collection
7.2 Tail vein blood collection
7.3 Cardiac puncture blood collection
7.4 Posterior vena cava
7.5 Orbital sinus8. Animal Experiments on the Cardiovascular System
8.1 Obtaining electrocardiogram (ECG) and blood pressure (BP) signals8.2 Myocardial ischemia induction
9. Animal Experiments on Ulcerative Colitis
9.1 Ulcerative colitis induced by 2,4,6-Trinitrobenzenesulfonic acid (TNBS)
9.2 Chronic ulcerative colitis induced by Dextran Sodium Sulfate (SSD) 2%9.3 Colitis with recurrence (Chronic)
10. Experiments on Anti-Bacterial Activities
10.1 Antibacterial activity by in vitro assays
10.2 Animal Experiments on antibacterial activity11. Experiments on Anti-Fungal Activities
11.1 Agar Disc Diffusion Method11.2 Minimum Inhibitory Concentration (MIC)
11.3 Sorbital test11.4 Neurospora crassa assay
11.5 Effect of Ergosterol on MIC
11.6 Test of ?1,3-D-Glucan Synthase (GS)
11.7 Virulence Factors Inhibition of Candida albicans
11.8 Germinative Tube Inhibition Test
11.9 Biofilm assay
11.10 Time-kill studies
11.11 Determination of Antifungal Activity by in vivo
12. Animal Experiment on Wound Healing Activity
12.1 Excision wound healing model
12.2 Incision wound healing model
12.3 Evaluation of the effect of the extract on migration/proliferation in fibroblasts
12.4 Surgical wound infected in rats (in vivo)
13. Animal Experiments of Anti-inflammatory activities
13.1 Inflammatory Paw edema
13.2 Lipopolysaccharide (LPS)-induced peritonitis
13.3 Murine Model of Immediate Hypersensitivity Induced By OVA
13.4 Air pouch inflammation model
13.5 Cotton pellet inflammation model
14. Animal Experiment on Anti-Nociceptive Activities
14.1 Abdominal constriction by acetic acid
14.2 Formalin test 2.5%
14.3 Hot plate Test
15. Experiments on Anti-Oxidant Activities
15.1 DPPH free radical scavenging activity
15.2 Hydrogen peroxide radical scavenging activity
15.3 Free radical nitric oxide scavenging activity
15.4 Ferric Reducing Antioxidant Power assay
15.5 Determination of superoxide dismutase (SOD)
15.6 Glutathione peroxidase (GPx) activity
15.7 Determination of catalase (CAT)
15.8 Determination of reduced glutathione (GSH)
15.9 Determination of malonaldehyde (MDA)
15.10 Total protein dosage
15.11 Test of oxidative degradation of deoxyribose
16. Animal Experiments on Hepatoprotective Activities16.1 Acute liver damage by paracetamol
16.2 Treatment of acute ethanol-induced injury
16.3 The induction of liver damage by carbon tetra chloride (CCl4)
17. Animal Experiments on Anti-Diarrheal Activities
17.1 Gastric emptying
17.2 Intestinal transit
17.3 Enter pooling induced by castor oil
17.4 Microvascular permeability
17.5 Activity on the H+/K+ pump – ATPase
18.1 Stress Induced experiment
18.2 Elevated Cross Maze (ECM)
18.3 Open Field Test (OFT)
18.4 Object Recognition Test (ORT)
18.5 Murinometric evaluation
19.1 Cytotoxicity test
19.2 &nbs
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