Apoptosis and Cancer Methods and Protocols

This volume discusses methods used in the fields of molecular and cellular biology for detecting and studying cell death, especially in cancer and cancer therapy. Chapters in this book cover topics such as non-destructive, real-time Annexin V apoptosis assay; assessment of the immune response to tumor cell apoptosis and efferocytosis; mass cytometry assessment of cell phenotypes and signaling states in human whole blood; cell cycle analysis of ER stress and autophagy; and simultaneous detection of inflammasome activation and membrane damage during pyroptosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Apoptosis and Cancer: Methods and Protocols is a valuable resource and practical guide for both novice and expert researchers looking into the “meaning of death.”

Preface...
Table of Contents...
Contributing Authors...

1. A Non-Destructive, Real-Time Annexin V Apoptosis Assay
Andrew L. Niles and Kevin R. Kupcho 

2. Cell Death Analysis in Cancer Spheroids from a Microfluidic Device
Julie Lafontaine, Elena Refet-Mollof, Ouafa Najyb, Thomas Gervais, and Philip Wong

3. Apoptosis Detection by Quantification of Cell Debris in Bright-Field Microscopy Images
Magnus Ölander and Per Artursson

4. Accurate Enumeration of Apoptotic Cancer Cells Using Flow Cytometry
David Diaz, Hugo Barcenilla, Alfredo Prieto, Jorge Monserrat, and Melchor Alvarez-Mon

5. Assessment of the Immune Response to Tumor Cell Apoptosis and Efferocytosis
Thomas Werfel

6. Quantitative Analysis of Apoptosis and Necrosis in Live Cells Using Flow Cytometry
Shivanshu Kumar Tiwari, Aswathy Sivasailam, Thomas M. Roshan, Prakash R. Pillai, S.V. Surabhi, Tilak Prasad, and T. R. Santhoshkumar

7. A Fluorescence-Microscopic System for Monitoring the Turnover of the Autophagic Substrate p62/SQSTM1
Hongzhong Jin, Qi Wu, Guido Kroemer, and Oliver Keep

8. Single-Cell Profiling of the Intrinsic Apoptotic Pathway by Mass Cytometry (CyTOF)
Tania Tan, Daniel H.D. Gray, and Charis E. Teh

9. High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens using Fluorescent Barcoding
Stanislav Drápela, Radek Fedr, Ondrej Vacek, Ján Remšík, and Karel Soucek

10. Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
Natalia Sigal and Holden T. Maecker

11. Quantification of Neutrophils Undergoing NET Formation and Distinguishing Mechanisms of Neutrophil Cell Death by Use of a High-Throughput Method
Shuichiro Nakabo, Mariana J. Kaplan, and Sarthak Gupta

12. Profiling of Protein Carbonylations in Ferroptosis by Chemical Proteomics
Ying Chen and Chu Wang

13. Cell Cycle Analysis of ER Stress and Autophagy
A. Popat, A.A. Patel, and G. Warnes

14. Proximity Ligation In Situ Assay to Monitor Autophagy-Related Protein Interactions and Autophagy in Cancer Cells
Paul Peixoto, Michaël Guittaut, and Eric Hervouet

15. Simultaneous Detection of Inflammasome Activation and Membrane Damage During Pyroptosis
Andreas B. den Hartigh and Susan L. Fink

16. Cancer and Apoptosis
Gul-e-Saba Chaudhry, Abdah Md Akim, Yeong Yik Sung, and Tengku Sifzizul Tengku Muhammad

Subject Index List…