Preface…
Table of Contents…
Contributing Authors…
1. An Overview of the Current ADC Discovery Landscape
L. Nathan Tumey
2. Pushing the Envelope: Advancement of ADCs Outside of Oncology
Michael J. McPherson and Adrian D. Hobson
3. Conjugations to Endogenous Cysteine Residues
Durgesh V. Nadkarni
4. Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies
Pragya Adhikari, Neelie Zacharias, Rachana Ohri, and Jack Sadowsky
5. Transglutaminase-Mediated Conjugations
Yasuaki Anami and Kyoji Tsuchikama
6. Click Chemistry Conjugations
Tak Ian Chio and Susan L. Bane
7. Utilizing Solid-Phase to Enable High-Throughput, Site-Specific Conjugation and Dual Labeled Antibody and Fab Conjugates
Sujiet Puthenveetil
8. Bridged Cysteine Conjugations
Mark Frigerio, Joao Nunes, and Matthew Bird
9. Antibody Conjugations via Glycosyl Remodeling
Hanna Toftevall, Helén Nyhlén, Fredrik Olsson, and Jonathan Sjögren
10. ADC Analysis by Hydrophobic Interaction Chromatography
Ryan Fleming
11. Two-Dimensional Liquid Chromatography Coupled to High Resolution Mass Spectrometry for the Analysis of ADCs
Soraya Chapel, Florent Rouvière, Morgan Sarrut, and Sabine Heinisch
12. Drug Loading and Distribution of ADCs after Reduction or IdeS Digestion and Reduction
Elsa Wagner-Rousset, Olivier Colas, Yannis François, Sabine Heinisch, Davy Guillarme, Sarah Cianférani, and Alain Beck
13. Analysis of ADCs by Native Mass Spectrometry
Oscar Hernandez-Alba, Anthony Ehkirch, Alain Beck, and Sarah Cianférani
14. High Resolution Characterization of ADCs by Orbitrap LCMS
Jintang He, Surinder Kaur, and Keyang Xu
15. Conjugation Site Analysis by MS/MS Protein Sequencing
Linjie Han, Yanqun Zhao, and Qunying Zhang
16. Conjugation Site Analysis of Lysine-Conjugated ADCs
Hua Sang, Ning Wan, Gaoyuan Lu, Yang Tian, Guangji Wang, and Hui Ye
17. Characterization of ADCs by Capillary Electrophoresis
Wenjing Ning and Yanqun Zhao
18. Characterization of the Primary Structure of Cysteine-Linked Antibody-Drug Conjugates using Capillary Electrophoresis with Mass Spectrometry
Josiane Saadé, Rabah Gahoual, Alain Beck, Emmanuelle Leize-Wagner, and Yannis-Nicolas François
19. Purification of ADCs by Hydrophobic Interaction Chromatography
Calvin L. Becker, Robert J. Duffy, Jorge Gandarilla, and Steven M. Richter
20. Detection and Removal of Small-Molecule and Endotoxin Contaminants in ADC Preparations
Jeffery Casavant, Anokha S. Ratnayake, Sujiet Puthenveetil, and L. Nathan Tumey
21. Physical Stability Studies of Antibody Drug Conjugates (ADCs) Under Stressed Conditions
Yilma T. Adem
22. Biophysical Methods for Characterization of Antibody Drug Conjugates
Vamsi Krishna Mudhivarthi and Jianxin Guo
23. Determination of ADC Cytotoxicity in Immortalized Human Cell Lines
Shengjia Wu and Dhaval K. Shah
24. LC/MS Methods for Studying Lysosomal ADC Catabolism
Andrew J. Bessire and Chakrapani Subramanyam
25. Assessing ADC Plasma Stability by LC/MS Methods
Cong Wei
26. Determination of ADC Concentration by Ligand-Binding Assays
Hsuan-Ping Chang and Dhaval K. Shah
