Activity-Based Proteomics Methods and Protocols

This volume comprehensively covers techniques that use activity-based proteomics to investigate biological functions of proteins such as lipolytic enzymes, serine hydrolases, chitinases, and glycoside hydrolases. The chapters in this book cover a wide range of protocols and an overview on the application of activity-based proteomics profiling to study deISGylating enzymes, serine hydrolases, monooxygenases, ubiquitin E3 ligases, and reactive cysteine in various organisms, including bacteria, viruses, and mammalian cells and tissues. Furthermore, this book provides updated, comprehensive protocols for utilizing activity-based proteomics profiling to discover new enzymes, detect novel modifications, and identify new drug therapy targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble3.shooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Activity-Based Proteomics: Methods and Protocols is a valuable resource for all researchers who are interested in applying the latest technologies in this advancing field.

Activity-Based Protein Profiling (ABPP) of Cellular DeISGylating Enzymes and Inhibitor Screening.-  Integrated Multi-Omics Approaches for Studying Rare Genetic Diseases.- Activity-Based Protein Profiling of Serine Hydrolases in Bacteria: Methods and Protocols.- Profiling Ligand-Induced Changes in Nuclear Localization Using Proximity Labeling-Coupled Chemoproteomic.- An Overview of Analytical Approaches to Cancer Proteogenomics.- Microplate-Based Enzymatic Activity Assay Protocol Powered by Activity-Based Probes.- Identifying Inhibitor Targets in Mycobacteria by Activity-Based Probe Profiling.- Application of Activity-Based Protein Profiling (ABPP) Strategy in the Identification of New Targets for Vascular Normalization and Binding Site Resolution.- Activity-Based Protein Profiling to Study Virus-Host and Viral-Induced MicroRNA-Host Interactions.- Activity-Based Protein Profiling of Active Host Cell Serine Hydrolases for Bioprocess Development of Therapeutic Proteins and Vaccines.- Synthesis and Application of Activity-Based Probes for Serine Proteases.-  A Chemical Proteomics Workflow to Profile Cellular Target Engagement of Kinase Inhibitors.- Isolation of Exosome from Senescent Preadipocytes for Mass Spectrometric Analysis.- Identification of Anticancer ROS Targets by Cysteine Reactivity Protein Profiling.- A Comprehensive Data Processing Pipeline for Phosphoproteomics in Viral Infection Studies.- Activity-Based Protein Profiling of Bacterial Monooxygenases.- Activity-Based Profiling of Reactive Nucleophilic Cysteine Sites from Tissue Proteomes for Drug Discovery Applications.- Activity-Based Protein Profiling for Functional Cysteines and Protein Target Identification.- System-Wide Profiling of Ubiquitin E3 Ligase Activities with Quantitative Proteomics and Bioinformatics Analysis.- Recent Overview of Protein Palmitoylation and Profiling Methodologies.