PRINCIPLES OF FLUORESCENCE SPECTROSCOPY

The third edition of the established classic text reference, Principles of Fluorescence Spectroscopy, will enhance upon the earlier editions' successes. Organized as a textbook for the learning student or the researcher needing to acquire the core competencies, Principles of Fluorescence Spectroscopy, 3e will maintain the emphasis on basics, while updating the examples to include recent results from the literature. The third edition also includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. This full-color textbook features the following: - Problem sets following every chapter - Glossaries of commonly used acronyms and mathematical symbols - Appendices containing a list of recommended books which expand on various specialized topics - Sections describing advanced topics will indicate as such, to allow these sections to be skipped in an introductory course, allowing the text to be used for classes of different levels Principles of Fluorescence Spectroscopy, 3 rd edition, is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine. TOC:Introduction to Fluorescence.- Instrumentation for Fluorecence Spectroscopy.- Fluorophores.- Time-Domain Lifetime Measurements.- Frequency-Domain Lifetime Measurements.- Solvent and Environmental Effects.- Dynamics of Solvent and Spectral Relaxation.- Quenching of Fluorescence.- Mechanisms and Dynamics of Fluorescence Quenching.- Fluorescence Anisotropy.- Time-Dependent Anisotropy Decays.- Advanced Anisotropy Concepts.- Energy Transfer.- Time Resolved Energy Transfer and Conformational Distributions of Biopolymers.- Energy Transfer to Multiple Acceptors, in One-, Two- or Three-Dimensions.- Protein Fluorescence.- Time-Resolved Protein Fluorescence.- Multiphoton Excitation and Microscopy.- Fluorescence Sensing.- Novel Fluorophobes.- DNA Technology.- Fluorescence Lifetime Imaging Microscopy.- Single-Molecule Detection.- Fluorescence Correlation Spectroscopy.- Radiative Decay Engineering: Metal-Enhanced Fluorescence.- Radiative Decay Engineering: Surface Plasmon-Coupled Emission.- Appendix I. Corrected Emission Spectra.- Appendix II. Fluorescent Lifetime Standards.- Appendix III. Additional Reading.- Answers to Problems.- Index.