Cell Culture and Upstream Processing

Upstream processing refers to theproduction ofproteinsby cells genetically engineered to contain the human gene which will express the protein of interest. The demand for large quantities ofspecific proteinsis increasing the pressure to boost cell culture productivity, and optimizing bioreactor output has become a primary concern for most pharmaceutical companies. Each chapter in Cell Culture and Upstream Processing is taken from presentations at the highly acclaimed IBC conferences as well as meetings of the European Society for Animal Cell Technology (ESACT) and Protein Expression in Animal Cells (PEACe) and describes how to improve yield and optimize the cell culture production process for biopharmaceuticals, by focusing on safety, quality, economics and operability and productivity issues. Cell Culture and Upstream Processing will appeal to a wide scientific audience, both professional practitioners of animal cell technology as well as students of biochemical engineering or biotechnology in graduate or high level undergraduate courses at university.

1. Cell Line Development and Culture Strategies: Future Prospects to Improve Yields 2. Use of DNA Insulator Elements and Scaffold / Matrix-Attached Regions for Enhanced Recombinant Protein Expression 3. Targeted Gene Insertion to Enhance Protein Production From Cell Lines 4. Recombinant Human IgG Production From Myeloma and Chinese Hamster Ovary Cells 5. Cell Culture Media Development: Customization of Animal Origin-Free Components and Supplements 6. Post-Translational Modification of Recombinant Proteins 7. Metabolic Engineering to Control Glycosylation 8. An Alternative Approach: Humanization of N-glycosylation Pathways in Yeast 9. Perfusion or Fed-Batch? A Matter of Perspective

Michael Butler-Associate Dean of Science; Professor of Cell Technology, Department of Microbiology, School of Science, University of Manitoba, Winnipeg, Canada